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Endofectin max转染试剂

http://www.biopike.cn/news/320707.html WebTransfection-mediated cytotoxicity can mask the true phenotype of a target gene being studied, so minimizing the amount of reagent used in your transfections is a critical factor for successful RNAi experiments.. Lipofectamine RNAiMAX Transfection Reagent gives maximal knockdown and excellent cell viability across a 10-fold concentration range of …

Secrete-Pair Gaussia Luciferase Assay Kit Genecopoeia

WebWhether it's raining, snowing, sleeting, or hailing, our live precipitation map can help you prepare and stay dry. WebNov 20, 2024 · For 1 μg of total plasmid DNA, dilute 3 μl of EndoFectin Max in 50 μl DMEM. The weight–volume ratio of DNA/EndFectin is 1:3. If the EGFP and mCherry constructs are cotransfected with integrin, the total amount of EndoFectin needs to be adjusted accordingly. 5. Mix the diluted DNA and EndoFectin and incubate at room … struggle is the meaning of life aga khan https://recyclellite.com

Sinofection® 转染试剂-义翘神州 - Sinobiological

WebsiRNA转染步骤—悬浮细胞. 6孔板实验为例:. 1、转染的当天,收集细胞离心,用含FBS的培养基重悬。. 2、 在250μl的无血清生长培养基中加入100 pmole siRNA,柔和混匀。. 3、 Lipofectamine RNAiMAX使用前要先混匀,然后在250 μl生长培养基中加入5μl的Lipofectamine RNAiMAX进行 ... WebEndoFectin™ Expi293(为Expi293F悬浮细胞进行优化设计),货号:EF007,专注转染悬浮细胞293F,转染效率极高. § 特别为Expi293F悬浮细胞进行优化设计,配合Expi293F悬浮细胞使用,可提高实验效率; § 与 … WebGenecopoeia endofectin max transfection reagent Endofectin Max Transfection Reagent, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more struggle is the father of all things

送京卡!EndoFectin™ 转染试剂 - 派克生物 - 博奥派克

Category:GeneCopoeia - EndoFectin™ Transfection Reagents 易 …

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Endofectin max转染试剂

转染试剂—DNA、RNA和蛋白质 Thermo Fisher Scientific …

WebProduct Description. EndoFectin™ Max is a new lipid-based transfection reagent. This versatile transfection reagent has been tested and optimized for highly efficient … WebLipofectamine™ RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. Lipofectamine™ RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine™ RNAiMAX Transfection Reagent's high-efficiency ...

Endofectin max转染试剂

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WebDOTAP脂质体转染试剂 >99% (TLC), liquid, suitable for transfection; CAS Number: 144189-73-1; Synonyms: DOTAP硫酸甲酯,脂质体转染试剂,dotap,转染试剂,dotap,N-(2,3-Dioleoyloxy-1-丙基)三甲胺甲基硫酸甲酯,转染试剂; find Roche-11202475001 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich WebExFect Transfection Reagent 是一种基于生物可降解的高分子树枝状聚合物的转染试剂,适用于多种细胞的DNA 转染。. 由于所使用的活化树枝状聚合物具有均一的大小和确定的形状,因此可实现高重复性的转染结果。. ExFect Transfection Reagent 携带DNA 进入细胞 …

Web核酸转染试剂. 转染试剂. Lipofectamine™ RNAiMAX 转染试剂. See the new Lipofectamine® RNAiMAX Transfection Reagent protocol —now optimized for efficiency, viability and … WebDec 19, 2024 · Methods:we grew mouse primary renal tubular epithelial cells to approximately 50% confluence, transfected using EndoFectin™ Max (GeneCopoeia, China). H/R was used to mimic IRI in vitro. Briefly, cells were incubated in glucose-free medium in a tri-gas incubator (94% N2, 5% CO2 and 1.0% O2) at 37 °C for 6 hours. …

WebJul 7, 2016 · EndoFectin™ Max、Lipo2000分别以FLuc表达质粒转染几种低转染率细胞,通过检测荧光素酶的表达活性比较转染效率。 图2. EndoFectin™ Max、Lipo2000分别对Hela细胞进行转染操作,通过计算转染后的细胞存活率比较细胞毒性。 WebEndoFectin™ Max GeneCopoeia现推出的EndoFectin™ Max是基于脂质体转染原理的转染试剂,该试剂具有多种细胞的广泛通用性,经优化设计,在多种常用细胞系中表现优 …

WebGeneCopoeia致力于人类基因组和基因功能研究的新技术与新材料的开发,建立了人类全长基因开放阅读框(ORF)、穿梭克隆库和表达克隆库,并以此为基础建立高通量蛋白质表 …

WebEndoFectin™ Max transfection reagent (1 mL) $295: Download: EF014: EndoFectin™ Max transfection reagent (3 mL) $589: EF001: EndoFectin™ Lenti transfection reagent (1 mL) $245: ... EndoFectin™ Expi293 transfection reagent (3 mL) $640 . Additional related clones. GLuc-ON™ Promoter Reporter Clones; struggle jennings outlaw lyricsWeb抱歉了,很久没来更新了,对转染试剂,再来做一期常见问题的归纳,希望能帮到大家。如果有我没涉及到的问题,大家可以留言,我再补充。 一、Hieff Trans™ Liposomal Transfection Reagent 脂质体核酸转染试剂Q: … struggle leads to successWebApr 1, 2024 · Donor DNA was cotransfected with either one of two guide RNA (gRNA) vectors or with scrambled control using EndoFectin Max Transfection Reagent (GeneCopoeia). Equilibrate gRNA vectors (1 and 2 each 5 μg) and donor DNA (5 μg) and endofectin were diluted in 250 μL of Opti-Mem I (Invitrogen) and incubated for 20 … struggle jennings mother and fatherWebEndoFectinTM-MAX 转染试剂广泛适用于常见细胞系, 如 HEK-293、 HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。. 该试剂即使在有血清存 … struggle jennings outlaw shit lyricsWebThe City of Fawn Creek is located in the State of Kansas. Find directions to Fawn Creek, browse local businesses, landmarks, get current traffic estimates, road conditions, and … struggle is the way to successWebCHO细胞的转染试剂。 Endofectin™-Max–最广谱的转染试剂,甚至适用于粘连细胞系和悬浮细胞系 应用不同的转染试剂将表达EGFP的质粒转染导人胚肾293T 细胞,转染20小时 … struggle meals beer can chickenWebAug 2, 2024 · ratio) into Huh-7 cells by chemical method using EndoFectin Max transfection reagent (Genecopoeia, #EF013) as described earlier (8, 10, 27). Huh-7 cells were seeded in T75 flasks and allowed to grow until they reached 80%–90% confluence. Cells were trypsinized, washed with OptiMem media, and 106 cells were resuspended in 5 ml of … struggle meals chicken thighs