H2b-rfp
WebMar 26, 1998 · The H2B–GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. Using confocal microscopy, H2B–GFP allowed high-resolution imaging of both mitotic chromosomes and interphase chromatin, and the latter revealed various chromatin condensation states in live cells.
H2b-rfp
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WebAt this time, the H2B.RFP-labeled CyMN.2 sample was also diluted ten-fold in series to create a standard curve. qPCR reactions were carried out using the DNA samples (100 ng per reaction), a TaqMan Gene Expression Master Mix (4369016, ThermoFisher), and amplicon-specific primer/probe sets (RFP and primate-specific AluYB8 (internal control ... WebThe Histone H2B-GFP expressing HeLa cell line is a useful cell model for imaging and analysis of chromosome dynamics. Histone H2B-GFP expressing HeLa Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS Data Sheet Key Applications: Cell Culture, Cell Based Assays SCC117
WebSep 3, 2013 · We expressed H2B-RFP under the control of truncated or mutated CKX2 promoters (Fig. 2 A and C) in transgenic plants and analyzed the RFP fluorescence in endosperm. Even when the promoter was reduced to a fragment of 395 bp, the pattern of H2B-RFP was confined to micropylar endosperm similar to that observed when we used … WebApplications Services Help and Support Popular Contact Us Quick Order Order Status and Tracking Documents and Certificates Search All Order Status Quick Order Sign in Error: …
WebApr 21, 2024 · Subcellular localization of VNS-YFP and RFP-H2B in leaf cells was observed with a confocal laser scanning microscope FLUOVIEW FV10i (Olympus) equipped with the filter units for YFP (Ex. 480 nm; Em. 527 nm) and RFP (Ex. 580 nm; Em. 601 nm), respectively. All images were captured by a digital camera DP72 (Olympus). Results and …
WebOct 12, 2010 · Download : Download high-res image (2MB) Download : Download full-size image Figure 2. GATA23 Reveals a Nuclear Migration Event (A) Confocal live imaging of the nuclear migration event in the XPP of a wild-type root using a p 35S ::FH6-GFP × p 35S ::H2B-RFP × p DR5 ::NLS-GFP triple marker line. molly mcbride live 5 newsWebThe histone H2B-RFP signal shows interphase nuclei, with the lower nuclei connected by a strand representing a segregation defect. Additional cell types and Hard-to-transfect cell types: (See Figure 2 in datasheet) Primary HUVECs were transduced at an MOI of 20. The image depicts an irregular interphase nucleus. hyundai school tripsWeb5.21.12.1.2 Histone ubiquitination and histone methylation. H2B monoubiquitination is an obligatory step in Lys4 H3 and Lys79 H3 methylation in yeast as well as higher eukaryotes (Figure 10). The connection between monoubiquitination and H3 methylation functions unidirectionally: deletions and mutations that block H2B monoubiquitination reduce ... hyundai schomp auroraWebIn addition to its utility for studying normal mitosis, histone H2B-GFP has been utilized to define mechanisms for asymmetric inheritance of oncogenic double minute chromosomes. Monitoring histone H2B-GFP also permits … hyundais cheapWebLet’s Draw More Eyes to Your Brand. Get the breakout visibility that leads to more traffic, more engagement, and more leads! Contact us and we’ll create an event experience that … hyundai schenectady nyWebPlease note that H2B-DsRed runs at a higher molecular weight than it is supposed to (~ 2kb instead of ~1 kb) on an agarose gel. Both the original vector and the Entry vector containing the H2B-RFP show the same … hyundai school trip fundingWebOct 18, 2024 · Pgk-H2B-RFP expression marks transduced EpSCs. Lines denote the dermo-epidermal border. Scale bars, 50 μm. c, Differences between transcriptomes of day 6 inflamed or day 30 post-inflamed … molly mcbride lambright seattle