2024 Maize single cell sequencing

Maize single cell sequencing

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August undefined, 2024

Web2.3 Single-cell library preparation and sequencing Six thousand cells per sample were targeted for capture. The cell suspension of each sample was run in the Chromium Controller with appropriate reagents to generate single-cell gel bead-in-emulsions (GEMs) for sample and cell barcoding. WebApr 7, 2024 · The structure of the maize kernels plays a critical role in determining maize yield and quality, and high-throughput, non-destructive microscope phenotypic characteristics acquisition and analysis are of great importance. In this study, Micro-CT technology was used to obtain images of maize kernels. An automatic CT image …

Advances and applications of single‐cell omics technologies in plant

WebMay 4, 2011 · Sequences after the maize APPLE mapping population (276 RILs) where collected in sixth lanes of a single ablauf cell at 48-plex. On average, 2,090 Mbp of DNA sequence data were collected per lane. WebApr 14, 2024 · Perhaps one of the most revolutionary next generation sequencing technologies is single-cell (SC) transcriptomics, which was recognized by Nature in 2013 as the method of the year. SC-technologies delve deep into genomics at the single-cell level, revealing previously restricted, valuable information on the identity of single cells, … boon chiang hainanese chicken rice

Single-cell RNA sequencing of developing maize ears facilitates ...

WebNational Center for Biotechnology Information WebJan 13, 2024 · A single-cell-resolution map of the maize root meristem, consisting of 14,775 high-quality sequenced cells, identified 21 cell clusters, which were then spatially mapped back to the... WebSingle-cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single-cell sequencing, we identified two subtypes of lens epithelial cells that ... has monkeypox been found in missouri

Uncovering hidden mitochondrial mutations in single cells

Single-cell RNA sequencing determines the evolutionary age of ...

Web1 day ago · A high-throughput single-cell single-mitochondrial genome sequencing technology known as iMiGseq has provided new insights into mutations of mitochondrial … Web2 days ago · The global Single Cell Sequencing market size is projected to grow from USD million in 2024 to USD million in 2029; it is expected to grow at a CAGR of Percent from 2024 to 2029. hasmonean kingdomWebApr 12, 2024 · Single-cell sequencing would allow you to examine and identify the components of each fruit (strawberries, oranges, kiwis, grapes). This gives a very clear picture of the genomes from each cell type and allows you to identify a potential gene, or target, that can only be found in the strawberries so that whatever therapy or drug you … has moneybag yo been to prison

"WebApr 6, 2024 · Here, we present a single-cell RNA (scRNA) sequencing and chromatin accessibility survey of rice radicles. By temporal profiling of individual root tip cells we … " - Maize single cell sequencing

Maize single cell sequencing

National Center for Biotechnology Information

WebMay 7, 2024 · The project, led by Alexandre Marand, applied single-cell sequencing technology to break up tissues and organs from maize, isolating thousands of individual cells to determine the regulatory DNA—the parts of DNA that control the genes that are important in different types of cells. WebDec 14, 2024 · Single-Cell Transcriptomic Approach for the Analysis of Maize Vegetative SAM Cells. Single-cell transcriptomic analyses of plant cells require the preparation of …

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WebApr 26, 2024 · We have developed a planta platform of single-nucleus RNA-sequencing by combined fluorescence-activated nuclei flow sorting, and used it to identify cell types in … WebMar 2, 2024 · Maize single cell sequencing dataset 1: Sample type: SRA : Source name: maize (B73) leaf 2: Organism: Zea mays: Characteristics: genotype: B73 inbred line tissue: V2 seedling leaf 2, distal 6cm: Treatment protocol: Protoplasts for scRNA-seq were generated from the last 6 cm of the distal portion of V2 leaf 2. For each pool of …

WebUsing single-cell genomics in six maize organs, we determined the cis - and trans -regulatory factors defining diverse cell identities and coordinating chromatin organization … WebApr 1, 2024 · Synergetic elongation of mesocotyl and coleoptile are crucial in governing maize seedlings emergence, especially for the maize sown in deep soil. Studying the genomic regions controlling maize deep-sowing tolerance would aid the development of new varieties that are resistant to harsh conditions, such as drought and low temperature …

WebDec 15, 2024 · In maize, single-cell sequencing profiling helped identify cells with unique characteristics on the adaxial side, enhancing the possibility of bioengineering the plant (Bezrutczyk et al., 2024). As single-cell transcriptomics is increasingly applied to several plant species, future research will indeed introduce it to crop species. WebApr 12, 2024 · Single-cell sequencing would allow you to examine and identify the components of each fruit (strawberries, oranges, kiwis, grapes). This gives a very clear …

WebFeb 21, 2024 · As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has become a powerful tool for describing cell subpopulation classification and cell heterogeneity by achieving high-throughput and multidimensional analysis of individual cells and circumventing the shortcomings of traditional sequencing for detecting the …

WebJan 4, 2024 · Request PDF Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery Crop productivity depends on activity of meristems that ... has moon knight finishedWebSingle-cell sequencing approaches reveal the intracellular dynamics of individual cells and answer biological questions with high-dimensional catalogs of millions of cells, including … boon chooWebDec 3, 2024 · Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the … boon choo flat rock ncWebApr 12, 2024 · Methodology/Principal finding. Amplicon-based sequencing of AI 535 (Low KRN) and AI 536 (High KRN) was undertaken for the gene fea 2 gene that codes for KRN in maize. Upon multiple sequence alignment of both sequences, A to T transversion at the 1311 position was noticed between Low KRN and High KRN genotypes resulting in … has moon knight finished filmingWebFeb 15, 2024 · A cell atlas of maize peels and leaf bases created by single-nucleus RNA sequencing provides insights into the molecular basis of stomatal movement and developm. ... We have developed a planta platform of single-nucleus RNA-sequencing by combined fluorescence-activated nuclei flow sorting, and used it to identify cell types in … boonchoo flat rock ncWeb1 day ago · A high-throughput single-cell single-mitochondrial genome sequencing technology known as iMiGseq has provided new insights into mutations of mitochondrial DNA (mtDNA) and offers a platform for ... boonchu kirchbergWebUsing single-cell genomics in six maize organs, we determined the cis - and trans -regulatory factors defining diverse cell identities and coordinating chromatin organization by profiling transcription factor (TF) combinatorics, identifying TFs with non-cell-autonomous activity, and uncovering TFs underlying higher-order chromatin interactions. boon choo thai express flat rock nc